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GrainGenes Sequence Report: NSFT03P2_Contig11938

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Sequence
CD492179
Contig
TaAffx.15685.1.A1_at
NSFT03P2_Contig11938
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
DB Remark
Locus Source: Triticum monococcum
Keyword
EST
Species
Triticum monococcum
Cultivar
DV92
Clone Library
Triticum monococcum DV92 early reproductive apex cDNA library
Tissue
Early reproductive apex
Developmental Stage
Seven week-old plants
Data Source
genbankRelease 136, Jun 15 2003
genbankUpdated Nov 2006
Title
WHE2479_H02_O03ZT Triticum monococcum DV92 early reproductive apex cDNA library Triticum monococcum cDNA clone WHE2479_H02_O03, mRNA sequence.
Strain
lab_host E. coli XLOLR
Clone
WHE2479_H02_O03
Remark
DB_xref: taxon:4568
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ).
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttgacttggttatgtgtgagattaaaatccgtg
caattttttttaatgttatgtcatctgactggaacttggttaagtctaaa
tagactgagacctagccacgcccagaaagaaaagaaaacttactgaactg
acacaaattacatccaaattttcaccttctgaccaacagggagtttgcaa
ccaccacattcggcacacgaaccacagtaaccaccccattcagcaacacc
agccaccgcatggagacgctggtgccgatttattcatgccaacacgcaaa
gttacatacacatgattattccccgaaggtcaagcaacacacacggccac
aagcaaaccaacctggggcatgcggttacagatacaggcagcaagagcag
tcaagctctactcatgagattgtgatcaccgggggcagcttgtctgacag
accgccgatcatcctctccgcgtgcactccccgttcgccggtgcgtaccc
ttacaacgtccgcgacgggtatcaagaatatcttcccatcgccaatttct
ccagttctggccttttcaattattttgtcaattactggctccacctgctc
cttgcagacgactatttccattttaactttagcaataaatgtatcttctg
aaagatctgacccttcataccgctccgtgggaccaccctgcgccccataa
cccgcacatccgacacggt

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