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GrainGenes Sequence Report: NSFT03P2_Contig2862

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Sequence	CD492037
Contig	TaAffx.43067.1.S1_at NSFT03P2_Contig2862
External Databases	Data at GenBank Data at EMBL Data at DDBJ
wEST map position	CD492037
DB Remark	Locus Source: Triticum monococcum
Keyword	EST
Species	Triticum monococcum
Cultivar	DV92
Chromosome	3BL
Clone Library	Triticum monococcum DV92 early reproductive apex cDNA library
Tissue	Early reproductive apex
Developmental Stage	Seven week-old plants
Data Source	genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title	WHE2460_C11_E22ZT Triticum monococcum DV92 early reproductive apex cDNA library Triticum monococcum cDNA clone WHE2460_C11_E22, mRNA sequence.
Strain	lab_host E. coli XLOLR
Clone	WHE2460_C11_E22
Probe	WHE2460_C11_E22
Remark	DB_xref: taxon:4568 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	tttttttttttttttttgagaaagatagaaaggcattaagcaatagaatt attagtcaaattccagtacaaagtatgactaccgcatcttaaaaatgcac atctaatagcttgtaaacatggccaaattgatgcctgctaatttcgcata tctttttccaagcaatacaatatttcttttcgcatacctacagtgcatat attgttcacaagagaatgagagaagaccatgatctccccagactctgcat gtaagaggagcaacagacctaattgaactacagcttgttgaccgaacatg tggcgggtcgtgggcgatgttggcgcctgtgctctcccaggtgaggcggt gaggaggcaacgacggcagcgcctgttgaatggcggccgtggagctccaa attggaggaggagagggtggtttccattcttgctccatcggttggtagcc atggagcctcaatttagggttgaatggatgttggattctgccatactcat cgagaaagataaatgcagtgtttcggttggactgaactcgggaactgtct agctgtcaaaatttgtgatgtgagcataaccatggacagatataaactga aaaccaatacagtttattttatacgagtacgctttg

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