GrainGenes Sequence Report: NSFT03P2_Contig15785
Sequence
CD491799
Contig
NSFT03P2_Contig15785
External Databases
Data at GenBank Data at EMBL Data at DDBJ
DB Remark
Locus Source: Aegilops speltoides
Keyword
EST
Species
Aegilops speltoides
Cultivar
F2 from 2-12-4-8-1-1-1-(1) x PI36909-12-811-(1 ) F2 from 2-12-4-8-1-1-1-(1) x PI36909-12-811-(1)
Clone Library
Aegilops speltoides wheat anther cDNA library
Tissue
Anther
Developmental Stage
Premeiotic anthers
Data Source
genbank Release 136, Jun 15 2003 genbank Downloaded 2008-2009
Title
WHE2215_C08_F15ZT Aegilops speltoides wheat anther cDNA library Aegilops speltoides cDNA clone WHE2215_C08_F15, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2215_C08_F15
Remark
DB_xref: taxon:4573 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in a growth chamber at the University of California, Davis (Akhunov). Premeiotic anthers were harvested, total RNA and poly(A) RNA were prepared, from each tissue and then pooled, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons , Zhang) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in a growth chamber at the University of California, Davis (Akhunov). Premeiotic anthers were harvested, total RNA and poly(A) RNA were prepared, from each tissue and then pooled, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttttcttttttttttttttagtttacagtaagctcattatataaag
atgataaccaccagatgatacccaccgaaatgataccaatgtgcagtaca
tttttacctaggctactcgaccaaataaatacggaatgagcgagatagga
agcaaggccgcctttcagaacaaaaacgcaaaaataaataacagggtaat
ataagtgttccttctaaccctccaaaaacaggttcaaatgcaagtatttc
ctagatcaacaaattgccttggtttgctcctcagatccaactaaagttaa
aaagataaaccagaaccggcatccacctccttgaaagcaactgtagacca
aactatagtctttgcactttgcaccagattcccaatgtgggcatgcccgg
atccgatctggagctctatgcctgggtctcgctcttgcgatc
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: NSFT03P2_Contig15785
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||