GrainGenes Sequence Report: CD491691
Sequence
CD491691
Contig
Ta.23482.1.A1_at NSFT03P2_Contig12004
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC350644
wEST map position
CD491691
NCBI UniGene
Ta.35953
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_001067159.1 Os12g0586600 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
5AS 5BS 5DS
Clone Library
CS wheat unstressed root tip cDNA library
Tissue
Root tip at 1.0 to 1.5 mm stage
Developmental Stage
Four-day old seedling
Data Source
genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title
WHE2854_C12_F24ZT CS wheat unstressed root tip cDNA library Triticum aestivum cDNA clone WHE2854_C12_F24, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2854_C12_F24
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions for four days. Root tips were excised and snap frozen (Ross and Gustafson) and total RNA was prepared at University of Missouri, Columbia. Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(- )phagemids in the TJ Close lab (Chin and Close) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions for four days. Root tips were excised and snap frozen (Ross and Gustafson) and total RNA was prepared at University of Missouri, Columbia. Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-)phagemids in the TJ Close lab (Chin and Close) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttttagttagaatactcacgaatttcacggtaa
acacttgaaatccaacataaggcactagtctgtctggactacatgcaccc
cattcctggaaagtctcggtcatatgccgatacggaataaaggggtcgaa
atgatcaggtttctgtttatcgatccacagaatatacatcctttcaggtc
tgaatcccagccagacatcaatcttacaggtctgctgacgagcaggccct
aagccctagcacagcaccagatacaaatgcttcttacacaacaatatgat
gcaaatctgccacactcgtaggctttaaaacttgtctagtgcagctcttc
tcaggcttgctctccatgcatcccaaaagaccctgcatggaggattcagg
tccacacattatggaatcggtcttttcgccacacctccttcacatggtgg
gttgcccttttcatcaggag
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: CD491691
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||