GrainGenes Sequence Report: NSFT03P2

GrainGenes Sequence Report: NSFT03P2_Contig12061

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Sequence

CD490915

Contig

Ta.26380.1.A1_at

NSFT03P2_Contig12061

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Transcribed locus, strongly similar to NP_001055195.1 Os05g0323900 [Oryza sativa (japonica cultivar-group)]'

Keyword

EST

Species

Triticum aestivum

Cultivar

Chinese Spring

Clone Library

Wheat etiolated seedling shoot normalized cDNA library

Tissue

Etiolated shoot

Developmental Stage

Five day old seedling

Data Source

genbank	Release 136, Jun 15 2003
genbank	Updated Nov 2006

Title

WHE3008_D09_H18ZT Wheat etiolated seedling shoot normalized cDNA library Triticum aestivum cDNA clone WHE3008_D09_H18, mRNA sequence.

Strain

lab_host E. coli DH10B

Clone

WHE3008_D09_H18

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. The normalization protocol used was modified from that of Soares'. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

ttttttttttttttttttaaaggttaagcggcctcaaactgaatactgaa
actgatacccaaagatatcttcaaacagtaatttcagctcaacataaaca
cgatgcctatgaacatgatttgcaagattttgctccaagtggaaaggcga
aaaactggaaacgcatggcaacaaattcaagcaaacatcgggcgctcagc
aggctttattaatggagaatagctctcctggccatcaccttgctcgaaag
gggtagtgccagnnacnacntggggagcagagaacagagtagcacgcaag
caggacgagcataatcagcaggccattgtgcattagtctacctcagcgaa
atgtacacaaggtccagcagctcgacaggtccatctttatttcacatgat
caaacagccgaaaaccagcacagatgagtattgtccattaagacaattca
ngcaagaacattttcgtacacctctccagcatatttccagttcatcacct
tccagatgttgttcaggtaatccggcctaacattcttgtactgcaggtag
tatgcatgctcccagacatcaatccccaacagcggaaccaagcttgcatc
tt


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