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GrainGenes Sequence Report: CD490584

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Sequence	CD490584
Contig	Ta.6842.2.S1_at NSFT03P2_Contig14306
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC316821
NCBI UniGene	Ta.54976
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_001046656.1 Os02g0313400 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Brevor
Clone Library	Brevor wheat dormant embryo cDNA library
Tissue	Seed embryo
Developmental Stage	Mature seed
Data Source	genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title	WHE2960_H12_O24ZT Brevor wheat dormant embryo cDNA library Triticum aestivum cDNA clone WHE2960_H12_O24, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE2960_H12_O24
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown to seed maturity under conditions favoring seed dormancy (L. Dohery at K. Walker_Simmons lab, Washington State University, Pullman, WA). Embryos were cut from mature dormant seed (Doherty). Total RNA was prepared from these embryos, polyA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Chin, Fenton). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	ttttttttttttttttttttttttagccgcaaaccaagagagctagcatg tgcagacggttaggatcctttgtatgagcttttgtgcttcccttgtgcac ccgacagtcacgcaccttgctggtacagaaaagctaacccagataaacag actcttgccactactctcgaatgggagaaaactgatccctttctcccttg gaatgtcgatttctgaaagatctcagcggtagccccaacctcttcctcgg cctcctctgccccgccctcttccactgcctctaccaccaccacggggtaa cccctcaaaagctctgttcacaagctgattgtgcattcctccatctcctc gccccttcttgtttaaaggggcattcccatactagcaggttgggtcctct tccctccagctgttgaagctgctggtttctttggttgttccatccatgat agagtaattttcttatctccaatgaataacggagatttcccatgcaatgg ctgtgacattgccaatatattattataggaccgcattgtcatcgtcgatt tttgctgatccgctttgattttagttttctcttcttctgtctttgctgaa gatattgccttgttgaaatctgccattccctgggttagtctcttcgaggc tgctcgaaccgtgtcttccgttccagttaacctctcaataaaatctttgt aatgttctgagaaatcctctccaagtctatctgatggctgtcc

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