GrainGenes Sequence Report: CD490455
Sequence
CD490455
Contig
Ta.27008.1.S1_at NSFT03P2_Contig13370
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC294749
NCBI UniGene
Ta.36833
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_001042770.1 Os01g0284500 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
BH1146
Clone Library
CS wheat aluminum-stressed root tip cDNA library
Tissue
Root tip at 1.0 to 1.5 mm stage
Developmental Stage
Seedling
Data Source
genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title
WHE2904_E06_J12ZT CS wheat aluminum-stressed root tip cDNA library Triticum aestivum cDNA clone WHE2904_E06_J12, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2904_E06_J12
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions, root tips were excised and snap frozen, total RNA was prepared at University of Missouri(Ross, Gustafson). Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK- phagemids in the TJ Close lab (Chin and Close) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions, root tips were excised and snap frozen, total RNA was prepared at University of Missouri(Ross, Gustafson). Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK- phagemids in the TJ Close lab (Chin and Close) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttacagtacgtgaaacagaagcaattttattgtct
caaacacgaacaaatgtaacatacacaacaacaaatcacctacacgctag
agatcacttgatgacttgaataccatttattcagtaacaaacaaacgatt
tattttcttccgaaacggatggagaggtgcccagggcagagtctccctac
ttgaacctagccttgacggcgtcgatgtcttcgttatcaaccctgaaggc
cttggccagcacgtcagttggcactggtggtgtggcagccaagagcgtgg
tggcgatggcctgggtgccctgaagctggctgttgaagccggcgataacg
gaggcggggcagtctcccctgttctgctggaagtgcacgagcccacgcgg
gaagacgaacacctctccgacggtgacggtcttagtgaagagcttgttgg
ccgtggtgatgaatccaacctcaagggttccctcggtgacgaagatgatc
tcggtggcgcgcgggtgggtgtgcggncgggttctgcccgcccaccgcgt
agtcgatgcgcgccatggacacgccgagcgtgttcaccccgggggaatga
ctgcacgttagccgcggtgacgaccgacccggccgggttgttggtggttg
ccggccttcttgagtccggcgaagaagaagtcatccgccgtgatgttcgc
cttgc
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: CD490455
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||