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GrainGenes Sequence Report: NSFT03P2_Contig8238

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Sequence
CD454870
Contig
Ta.28381.2.S1_a_at
NSFT03P2_Contig8238
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC301676
wEST map position
CD454870
NCBI UniGene
Ta.55436
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001059117.1 Os07g0195400 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
7BS
Clone Library
CS wheat heat stressed spike cDNA library
Tissue
Whole spike
Developmental Stage
Spikes at 5, 10, 15 and 20 days after anthesis
Data Source
genbankRelease 136, Jun 15 2003
genbankUpdated Nov 2006
Title
WHE1701-1704_K01_K01ZT CS wheat heat stressed spike cDNA library Triticum aestivum cDNA clone WHE1701-1704_K01_K01, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE1701-1704_K01_K01
Probe
WHE1701-1704_K01_K01
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Spikes at 5, 10, 15 and 20 days after anthesis were heat stressed under two conditions at Texas Tech University (D. Zhang in HT Nguyrn lab): (1) at 38 C for 4 hours and (2) 5 days of cyclic treatment of 38 C for 4 hours. Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttttttttttttttaaaaaagaaacagcaattttgcacagaaacaa
aatatatgtatacattcttcaaaacgtagggaaatcagctaaagttgaaa
attacacgtttcagaaagagatgaaagataaaagaagaacgcaactagca
aaacccgagaatgcgctcaacatggtgtgctacagattttgcaaggctgt
ccgctgcttccacggtagatgcctctgcgtatactcttaccacatcctca
gttccagatggccgcacaaagcatcgcccgtgagagtaattaacgacttc
cttatctatcaattcttgcaagccagatggttggcaaacttttcgttctg
catcggttgtaacaatagaagttcgatctttcactttaaccttaagttgt
ttacttggtagatcagtgtataggtcacaccaattctgaaatgaccatct
cttatgctgtaaaacagcctccaccaaaagcattccacttatagcatcac
ccactgcttgattaattaattgacttgttgctaccaatctcaacgcagct
tggtgctgtggagaacctgcagcttcagaaagcctagcagtcaaagactc
aagccgtgagacaaactcatcattgaatagcactgtcccgtgtccattgg
gctcaaagtagacaccaatatcata

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