GrainGenes Sequence Report: Ta.10100.2.S1_s_at
Sequence
CD454740
Contig
Ta.10100.2.S1_s_at Ta.25242.1.S1_at NSFT03P2_Contig3320
External Databases
Data at GenBank Data at EMBL Data at DDBJ
wEST map position
CD454740
NCBI UniGene
Ta.10100
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_001064561.1 Os10g0406100 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1AL 1BL 1DL
Clone Library
CS wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title
WHE2341_A10_A19ZT CS wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE2341_A10_A19, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2341_A10_A19
Probe
WHE2341_A10_A19
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A ) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttgtttcaggtgtggaatcaaatattatgaaacactatgca
caagtataacatcagtccttgattacaatgttctgcaaaaaaatatgtat
gggaatagcattgttccattatttaaatatatagggaaaaatgggcctta
aataaaagccatgtagtcgattattgttctcttacaaaaccacgtgggtg
gtttctgcagccttgcaagtttgcaactaaaattagttcttcagactctg
caggatatattctgcgtataggtccattgagtgctgaacggcaacctgtg
cagtgttcgatggcaaaaagtcattcaaagcaacctccttgttctcattc
ttcttgtaatcttcgaaaaagcgacggatttcagcaaggcggtggggaga
gagctccttaaggtcattgaaatgacggtactcgggatcatcaacgcaaa
ctgcaatgatcttatcatctttctcaccctgatcaatcatgggcataagc
ccaatagccttagctcgaagaaagcaaccaggaatgacgggttcctgcat
ca
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: Ta.10100.2.S1_s_at
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||