GrainGenes Sequence Report: CD454259
Sequence
CD454259
External Databases
Data at GenBank Data at EMBL Data at DDBJ
wEST map position
CD454259
NCBI UniGene
Ta.25907
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
4AL
Clone Library
CS wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title
WHE1770_C04_F08ZT CS wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE1770_C04_F08, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE1770_C04_F08
Probe
WHE1770_C04_F08
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A ) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttcaacaactcgtaattgttgaaaatggataacaa
tagatgcaatcatcattctctgtttacaaacaagcacacacatagtgggc
agcagcaaatggagagcaatcaatcatctctagaagaacagagtactaga
aagcaaagtaagtctgtattaaaaattgaaggtcttttcatcacgttgat
catctaatccattccatggggttccccaagaaccaaaattttcatctggg
ccaacaattgcaacctgtaaaccttctgagagctcaaaatcgcatcagaa
cttggtaatcccctccgttacactaaagccccaagatcaatgcactcatc
ttccattcgcagtgtagaaacgtcgaggtccctctcagcttcatcttgca
ccgtatcaaagttcaaaggccagcagcccttagagaacttggagtgctga
acgtgatggtcaattgcagcatttatggtgaacatgccaacaacgaaaag
gagctgaactgaccatttatggtgtgttcacagttcacactttagaacat
tcagtttccttttcaagcaccggatggctaatatgttttgttcacttcag
ctgcagaggcatgcaccctgctcagacctcatatacctgagagttgctcc
agc
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: CD454259
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||