GrainGenes Sequence Report: CD454189
Sequence
CD454189
Contig
Ta.25979.1.A1_at Ta.25979.1.A1_s_at NSFT03P2_Contig6737
External Databases
Data at GenBank Data at EMBL Data at DDBJ
wEST map position
CD454189
NCBI UniGene
Ta.25979
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, weakly similar to NP_001048560.1 Os02g0822500 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
2BL 2BS
Clone Library
CS wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title
WHE0990_H04_P08ZT CS wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE0990_H04_P08, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0990_H04_P08
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A ) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttttttttttttttttttttttttttttttttgaggggggggtggc
aaaaaaccgaaacacatccatgcataatggagcactcactgctagtcatg
actaattttgtcaatgctgctttttgacaaaatacacacattgcttttgc
ttccaaccatctcaaaccacacacatggacagcaatgtagcaagcaaacc
acatttccttgggtctttctgttgcattttttcttgcaggggagaaggaa
ggaaggaaggaaaaatgaatgaatttattggttgggttagttttccggga
agacggtgcggatgaaattgaggaagccctccgagtagaaccggggctcc
acgaccgagatcgacggggggttgtaattgatggacttgtaggcgtgctc
caccttcttcctcatgctgtacgcctgcaggatgtccatgatccctatgt
agagcaccacgtcgtacacctgagggagcgatttgccgccgtctttccct
tgtttcccttccttctccgcgcatgccggcatgttcct
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: CD454189
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||