Query (optional)   in Class  

GrainGenes Sequence Report: NSFT03P2_Contig17332

[Submit comment/correction]

Sequence
CD453978
Contig
Ta.3706.1.S1_at
NSFT03P2_Contig17332
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC333269
NCBI UniGene
Ta.23445
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, strongly similar to NP_001047033.1 Os02g0533900 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
CS wheat 5-15 DAP spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbankRelease 136, Jun 15 2003
genbankUpdated Nov 2006
Title
WHE0939_F08_L15ZT CS wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0939_F08_L15, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0939_F08_L15
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttgttggttgataggtctacattacagccaaag
caaatggtatatatatcttggaggtcaaagatgctgattctgccaattat
gatccacctattgattttcagtaactaaaatcagtaactgtaggggtacc
acctaaatgtttcaacaaatgcgatgcatcattattatattgctttgatc
cctcatcttcatacttgtaggagcaaatattctcttacatgacagtattt
tattaatgctctattggtggaaaatcaccgagtcgcaaacacggggatac
gttgcaaagaggcaaaccatttcttttgcagggggaagctagacttacgg
accctgtgttttcccagtatagcattctggagttcatgtcgtcttcataa
aagtggaacctaggatgatgatgatgtcttgccatccaacgtcagtagca
ctttgtatagatcaggacgcctgtccctgaatatcccccagccgtgtctc
gtagatttgatctcgtccaagtcaaactctgccacgagcacctcctcgtc
tttgtcatttgcaagcttcacgatttctccagttggccctgcgatgaatg
agttcccgtataatttt

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.