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GrainGenes Sequence Report: CD453908

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Sequence
CD453908
Contig
Ta.7200.1.S1_at
NSFT03P2_Contig6323
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC310874
wEST map position
CD453908
NCBI UniGene
Ta.7200
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, strongly similar to NP_001062410.1 Os08g0544900 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
5DL
7BL
Clone Library
CS wheat 5-15 DAP spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbankRelease 136, Jun 15 2003
genbankUpdated Nov 2006
Title
WHE0903_E01_J01ZT CS wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0903_E01_J01, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0903_E01_J01
Probe
WHE0903_E01_J01
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttttttttttttttttttagcaacaaaacaatt
aaatcaaaggtaccaagctaaaggaaaacaaaaatgcttgtactacacga
aggtttcagttcagctcaacacaaaaaataagctgaagagtgtgggggaa
acaactttaaaaaataatggcttatttgaaacttagctagactggaaatt
ctaaaaataaatacaacaaattgtacggatgatggctaaaaaaatgcacg
gtgaactaacagtatctgatgatatgccactagtgaccatttgcaatgcc
attgctgcacttgattttgccgtcaaaaatgccatcggttttcttttcaa
aaaaccccctgtacattgagtcaacttttgtaaggtaaaacgttcctggg
ggcaacaagcttatgtctttgcttgtttcaaaatcttttgccccaaatcg
gtgctccatcagcttcagtgtgtcaatgaacttcccaggtgaagtcacat
gtcttgattcaagctttgccgtcacatcaagcaaaaaagcaatgtttgct
aaattaaagggatgt

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