GrainGenes Sequence Report: Ta.9859.1.A1

WHE2165_E03_J05ZT Triticum turgidum L. var. durum (durum wheat) Langdon-16 whole plant cDNA library Triticum turgidum cDNA clone WHE2165_E03_J05, mRNA sequence.

Strain

lab_host E. coli SOLR

Clone

WHE2165_E03_J05

Remark

DB_xref: taxon:4571

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in a growth chamber at North Dakota State University (Kianian, Otto, Simons). Tissues collected from seven-day etiolated seedling leaf, stem, root and seed; leaf from plant at fourth leaf stage; spike from pre-anthesis through 20 days after anthesis; flag leaf; leaf and stem tissue from tillers. and root. Total RNA and poly(A) RNA were prepared from each tissue and then pooled, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

tttttttttttttttttttatggcagacaaacgtgcccttaagatatgtc
tctctcgtactccctccgtctgaaaatacttgtcctcgaaatggatcaaa
tggatgtatctataattaaaataagtctagatacatccatttttaggaca
agtatttccggacggagggagtagtatattcctgtttttttctcttttgg
cacaagagaggccttctaagacgaacggggagggagtactctacatcttg
aaatcggattatcaaaatacagtgctctatatttactcaatttgcatgtg
ccaagccctagggagggtgaagtcacttcgacgacttgccttgtgggcgc
agtcaagtttttgtgcagaggaaatcattgggtttccctatcctgatatt
ttgctcctgcaaatagctcgtgatagcgttcttttgccaccccttgaagc
tcttctgatcaagatgtcct


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