GrainGenes Sequence Report: CD453848

[Submit comment/correction]

Sequence

CD453848

Contig

Ta.3607.1.S1_at

NSFT03P2_Contig11214

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

wEST map position

CD453848

DB Remark

Locus Source: Triticum turgidum

Keyword

EST

Species

Triticum turgidum

Cultivar

Langdon-16

Chromosome

3AS

3BS

3DS

Clone Library

Triticum turgidum L. var. durum (durum wheat) Langdon-16 whole plant cDNA library

Tissue

All tissues

Developmental Stage

Different growth stages

Data Source

genbank	Release 136, Jun 15 2003
genbank	Updated Nov 2006

Title

WHE2158_A05_B10ZT Triticum turgidum L. var. durum (durum wheat) Langdon-16 whole plant cDNA library Triticum turgidum cDNA clone WHE2158_A05_B10, mRNA sequence.

Strain

lab_host E. coli SOLR

Clone

WHE2158_A05_B10

Probe

WHE2158_A05_B10

Remark

DB_xref: taxon:4571

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in a growth chamber at North Dakota State University (Kianian, Otto, Simons). Tissues collected from seven-day etiolated seedling leaf, stem, root and seed; leaf from plant at fourth leaf stage; spike from pre-anthesis through 20 days after anthesis; flag leaf; leaf and stem tissue from tillers. and root. Total RNA and poly(A) RNA were prepared from each tissue and then pooled, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

tttttttttttttttgaaattcacggcttgatcaataataagctatattt
ctcagaaaaaaaaaaaaaagctatatatccacactggtgaacgaacaaca
aatcaggaattaacaaacactgtcctgagtgcagcaaagacgcttcgtat
tcactagaggattcttcttatagatctacaactgtgagcctcctacataa
tttatcagatggagcagaggagcacagcttcgttttcaggaagtataggt
gtctgggagagtgaactccgagagaggcatcttgcctcggatcattgcgc
cggcgtttggcatgatgtgccagtggaggacaagctgaactttcttgccc
cgcagactggtcccctggtcagtcaggggatacttgctcttcacctccac
ttggacatttgcctcctctttgtcaggtattatcttgtcccataatgaaa
cctggttcagggaattctttgcggtttcatactccgccgtcacaaaggca
aagacctgctttgtgttccacgtgaaaagtgattccaaatttgcaaatag
actgaaggtcaaggtcaccttgtcattcccattgatctgtttgtggaatc
aatttcct


GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.