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GrainGenes Sequence Report: Ta.6326.1.A1_a_at

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Sequence
CD453804
Contig
Ta.6326.1.A1_a_at
NSFT03P2_Contig8327
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC307097
wEST map position
CD453804
NCBI UniGene
Ta.6326
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001066673.1 Os12g0429200 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
CS wheat salt-stressed crown cDNA library
Tissue
Crown
Developmental Stage
Adult plant
Data Source
genbankRelease 136, Jun 15 2003
genbankUpdated Nov 2006
Title
WHE2120_B04_D08ZT CS wheat salt-stressed crown cDNA library Triticum aestivum cDNA clone WHE2120_B04_D08, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2120_B04_D08
Probe
WHE2120_B04_D08
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA and poly(A) RNA were prepared from crown tissue, equal portions of RNA were pooled from the two treatments, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttttttttttttttttttttttttttttaggcg
gacttttccctgtccttatttcttcagttgtaggattatctttacaccaa
caccaagcacaaccatgggtcaagcgaaacactcccttacaccagtttgt
caccagcatcccgcatcaaatctcgtctcgaggttctttgggggccgatg
acagcgagcactcagcctcaactgccaaatccttggggacccctcggcat
ggttcaccaaacacatcattcgaaacactgattgcgcattcgttgttgcc
cacgcacgcctctgataccaagggaagagtactcaaagcatggcactttc
cttcggagaaattctggcaaccacctgatggagttccgtagctcgcaaat
gtaatcttagtgatcaaatgcccatcatcacaccgtaagcgcagttctgg
tgctatagtatcaaccaagacacgaccactggtaaggcgtgaccaaccaa
aaaggggaggataataactttctgatatccttgagcagattgttttggta
aaatgaacctccagtgatatctttgaagggtcaccccctgtt

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