GrainGenes Sequence Report: CD453729
Sequence
CD453729
Contig
Ta.4208.1.S1_a_at TaAffx.134015.1.S1_s_at NSFT03P2_Contig11474
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC292139
wEST map position
CD453729
NCBI UniGene
Ta.4208
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, weakly similar to NP_187456.1 SCPL27 (serine carboxypeptidase-like 27); serine carboxypeptidase [Arabidopsis thaliana]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1AS 1BS
Clone Library
CS wheat 20-45 DAP spike cDNA library
Tissue
Spike and seed
Developmental Stage
Adult plant
Data Source
genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title
WHE0856_C06_E12ZT CS wheat 20-45 DAP spike cDNA library Triticum aestivum cDNA clone WHE0856_C06_E12, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0856_C06_E12
Probe
WHE0856_C06_E12
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 20 DAP and seeds at 30 to 45 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 20 DAP and seeds at 30 to 45 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttttttttttttttagttaagaagcatctattgctttattgatgat
cactcttgttttctcacatcgaaattactatttgacttccatccctttgt
tgctaagccaccgtcgcattcttggtctggcctggcatgggctcgccctg
caggaagtaccgaaacagtacgagcgcttgccgcgggcgatgcaggggaa
cctcatggccggcacctctgacggacaccagtgtaaggcccttgtacacc
tggctccaaccgccgacctcctggtcgtcataccaagggtaccaactggt
agtagttggaagacccagaccgccgatggagtatcttgttgctgtcaagg
ggactaccgcgtccgtgtcaccgctgaacacccaaatcctgaggcccgct
gcaataagctccctgtaaatgggaagcatggacctcggagcatcatgcca
gtgggtattaatggtgtcgctgcaggtcgcccacgtgtagttcatggcgc
cggtgacgttggcgtggagagccatctgcacgtcccgccggttgtagtac
gccgtcgagtaccgttccgtgcacgggtcgtaggcct
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: CD453729
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||