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GrainGenes Sequence Report: CD453617

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Sequence	CD453617
Contig	NSFT03P2_Contig11543
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC284560
wEST map position	CD453617
NCBI UniGene	Ta.55224
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001056918.1 Os06g0167100 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	6AL 6BL 6DL
Clone Library	CS wheat vernalized crown cDNA library
Tissue	Crown tissue of seedling
Developmental Stage	Five-week old seedling
Data Source	genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title	WHE0821-0824_F01_F01ZT CS wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0821-0824_F01_F01, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE0821-0824_F01_F01
Probe	WHE0821-0824_F01_F01
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	ttttttttttttttttttggccggcagaataatttgattatccttgaatg taaaatacacatgcgtgaaaataattatgatgattccaaatattttacac tgctgtaactactatacactatatattgagacaatgatgggcaagccgga ctgtctgtttaagtgccctcaaatggggaacaggagccgaaaaagtataa caagagataagaatctcccctctgccccgaacacatgaaatgaacatagc tatcacaacaaaacataggttacacacaaatggcactatactccaggtgg aaaattgctgacttggccttgttccaacctgtcctcgccgttcactttgt aagacatccggatacgcattgcaagcggtttctgcccatgctgattattc gtgacactgaaaccttgtgtgactgaaccctttccattggctggaacaat gttaccgctagctggatccagtcgcaattgaatgaactttggaacagctg cctgaaaaatgaaatctgtataattgctagatgtcaaatttgtaaaagtg gcatggatggtagtctcttgcggctttccaggctgttttctgatatcaaa ggtgatcttcaaagttgcactctggaaagccgttattgacgggcgagtat cttcaagtccagaaacggatgtacttggtgacaaaccgt

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