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GrainGenes Sequence Report: Ta.572.1.S1_at

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Sequence
CD453518
Contig
Ta.572.1.S1_at
NSFT03P2_Contig17392
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC290900
NCBI UniGene
Ta.572
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001055434.1 Os05g0389500 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
CS wheat heat-stressed seedling cDNA library
Tissue
Leaf
Developmental Stage
Two-week old seedling
Data Source
genbankRelease 136, Jun 15 2003
genbankUpdated Nov 2006
Title
WHE0766_H07_P14ZT CS wheat heat-stressed seedling cDNA library Triticum aestivum cDNA clone WHE0766_H07_P14, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0766_H07_P14
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Two-week old seedling were heat treated at 37 C for 2 hr and cyclic heat treated at 37 C for 2 hr for two days at Texas Tech University (D. Zhang, H. Nguyen lab). The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttccaaaaacaaaaagtgtccagtgttctgggaaatgcataca
ctgataaatgcacatacctctttggccgaaaaccaaaaggcacatgtttc
ttcacattcataccaccacccacgcacaaaaggtaacaaagtataccgtt
atcaattgacaaattgcttctgaaaaacaacaaaaaaatgtggctgtgaa
atttattgcatgcaccacagccttatgattaaccctagactgaacccaga
ccatggacaacagttcgagggccatttgccgccagtcaaaaaatgcaacc
ggccaccctaggcactagctagctagctgctaattacctgaaaatcggca
ccgattggcaagtgatcactaggatggtggaagttgggcaggcctccttc
cacatccggggaaccccctcgcgggatccgaagtaggctactaggtttta
ttgaaccccc

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