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GrainGenes Sequence Report: CD453501

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Sequence
CD453501
Contig
Ta.25834.1.A1_at
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC316394
NCBI UniGene
Ta.25834
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, weakly similar to NP_001061231.1 Os08g0205100 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
CS wheat heat-stressed seedling cDNA library
Tissue
Leaf
Developmental Stage
Two-week old seedling
Data Source
genbankRelease 136, Jun 15 2003
genbankUpdated Nov 2006
Title
WHE0765_D06_H11ZT CS wheat heat-stressed seedling cDNA library Triticum aestivum cDNA clone WHE0765_D06_H11, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0765_D06_H11
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Two-week old seedling were heat treated at 37 C for 2 hr and cyclic heat treated at 37 C for 2 hr for two days at Texas Tech University (D. Zhang, H. Nguyen lab). The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttttttttttttttttttttagaggaagtatactttttaaggtttt
tgtgaataattaataaaatggttgcatgcatcgttcagatgcaaggccgg
aggtatatcctccttttctaaaaaaagaaacactcctctgaacctctata
actactcaagtgcacagtgtcaactcagaacaaaaataagtacctcttat
gtgtgagatattctctaagacgaccaatgatgtcttgcacctcgtgagcg
tgagttaactcctctttcatcccaagcttcaataggagaccaccgaggag
gcttgacatatcaggtctttgagaaactgataaaaatattgtacagctga
attgccctccgatcatatcatatacttgtttggcaagtgtagttttacca
agacctccaaatccaacaatagccactaccttgactttttcctgagtatc
tgccaaccaactcacaagctctttctttgggccatcaatacccacaagcc
ccgcttcctgcttgtagatcgccaacatccgagtatcgatgggaacgaca
cgagtggtagaattggcacaatcatcaatcttgtacctcatgcgtcgttc
atttgcttgcaaagcaagggccttgagctcttccatccgatcagcgatac
gatgacgcttccgcagcgtcttaagacgtttagccgtcttctt

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