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GrainGenes Sequence Report: CD453453

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Sequence	CD453453
Contig	Ta.28018.1.S1_at NSFT03P2_Contig17014
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC282259
wEST map position	CD453453
NCBI UniGene	Ta.48473
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Clone wlk4.pk0006.e8:fis, full insert mRNA sequence'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	3AS 3BS 3DS
Clone Library	CS wheat heat-stressed seedling cDNA library
Tissue	Leaf
Developmental Stage	Two-week old seedling
Data Source	genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title	WHE0753_B05_D09ZT CS wheat heat-stressed seedling cDNA library Triticum aestivum cDNA clone WHE0753_B05_D09, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE0753_B05_D09
Probe	WHE0753_B05_D09
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Two-week old seedling were heat treated at 37 C for 2 hr and cyclic heat treated at 37 C for 2 hr for two days at Texas Tech University (D. Zhang, H. Nguyen lab). The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	tttttttttttttttaacggcaactcacttcattcctggaagaacccaca agaccaaaccattcaagaattgtcccctaaatgcctcgcagagttgcaga agcttaaaactcaggctatacagtagcaaaatagtgcacaccatactaaa gatgcaaacccctacatagtagcctgataaaaaagacccagactcctcaa gtttgacgattatactcctcttacaaacgctgtgatattgatacccagga ccagttgcttccttgaaaatcttcagcttattggacccaccacaaccatg accttaccccacacaccgactagaccaaatggtgacaactgctgcgacaa gggaattaccaccattggtcaaatctgtattcagcccaagttcagatcag ggttgccaccttggccaaagtcaaaggcaacacctgtgggagcttccata gttgcccccatcgcatcatcttcctcctccatccagtaagcctcgagaat cttcacagccttctcatagatctcattgttatcatggctctgcaggttct caatcttttccaggccctctgcttcatcaatcatctgcgcatagacattc ccctccccagaagcacccaaattcttatcagtttctccaactttgagaat gtt

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