Query (optional)   in Class  

GrainGenes Sequence Report: CD453396

[Submit comment/correction]

Sequence
CD453396
Contig
NSFT03P2_Contig11566
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC312839
wEST map position
CD453396
NCBI UniGene
Ta.48442
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Clone wkm2c.pk005.e4:fis, full insert mRNA sequence'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
4AL
4BS
4DS
Clone Library
CS wheat heat stressed flag leaf cDNA library
Tissue
Flag leaf
Developmental Stage
Full tillering stage
Data Source
genbankRelease 136, Jun 15 2003
genbankUpdated Nov 2006
Title
WHE1655-1658_J24_J24ZT CS wheat heat stressed flag leaf cDNA library Triticum aestivum cDNA clone WHE1655-1658_J24_J24, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE1655-1658_J24_J24
Probe
WHE1655-1658_J24_J24
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants at full-tillering stage were heat stressed under four conditions: (1) 38 C for 2 hours, (2) 38 C for 4 hours, (3) 2 days of cyclic treatment of 38 C for 2 hours, (4) 2 days of cyclic treatment of 38 C for 4 hours, at Texas Tech University (D Zhang in Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttttttttttaatcaagtcggaaagattctgcc
gttgattttgttctcaactgacaagtggcatgtacataaccattccaatc
tgtcagtctagttgcatcttttatacttctactatagaaatatgtacatg
aataggtgtaaaaagtaaattacacaggacattgtcaatgctttccaaga
tgtaatgggattcctcaaaaaaaagttgtgatgggtaaattttatgtgtt
ccatgatgactaaatttgatgtgttccatgataacactatgcctgtaaat
atcctctcctcttggagaaatgccaataagtatgctggttaaagaaatcg
tgtacattaccatgttgcgtcagagtaggaaacatgcatatccagcaggt
gcgctccacacattcaggaaaacttggtccctcgatttccagaatgtcga
gggttgagtgtatcctgggaaacatgaaaatagaaatgcacactcccttc
gcaggcattatcctgttcaggttcaaagggactttctcagcccatatcgc
aacaatccaggccaaggggccttaagatcgttgcct

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.