GrainGenes Sequence Report: CD453220
Sequence
CD453220
Contig
NSFT03P2_Contig18531
External Databases
Data at GenBank Data at EMBL Data at DDBJ
DB Remark
Locus Source: Secale cereale (rye)
Keyword
EST
Species
Secale cereale
Cultivar
Blanco
Clone Library
Rye anther cDNA library
Tissue
Anther
Developmental Stage
Adult plant before anthesis
Data Source
genbank Release 136, Jun 15 2003 genbank Downloaded 2008-2009
Title
WHE1295-1298_F03_F03ZT Rye anther cDNA library Secale cereale cDNA clone WHE1295-1298_F03_F03, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE1295-1298_F03_F03
Remark
DB_xref: taxon:4550 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Anthers were harvested and pooled from early meiosis to late meiosis. The tissue, total RNA, and poly(A ) RNA were prepared (Butler, Ross and Gustafson) at University of Missouri, Columbia. A cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Anthers were harvested and pooled from early meiosis to late meiosis. The tissue, total RNA, and poly(A) RNA were prepared (Butler, Ross and Gustafson) at University of Missouri, Columbia. A cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttaatggcaaccatgccttataaccgaagataaag
gtaccaattatagcagtacaacagatatccacggccttaaaatataggcc
ggatcagatggaaagcctcaaaataatacagggagaacaagccgaaccat
taagtggttaagtgttgctttccgtaccatcaccgaatttggtctagttg
aagtcaggcttctcagggtaagtgcagccagactgctggatgataacgtt
tgctgcgtagcaaccggcctttacgcagtcctcgatgctcttcccttgaa
ccaactgagagaggaagcctccaacaaacgcatcaccagcgccattggtg
tcaacaagcttctccttgggcaataggatcacagggaatgttttcacctt
cccatcctcagccacaactactggatcagcaccctgagtaatcacagcaa
tcctcttttgtttcccagaagccagaggcagttgtgagatcttcaaagcg
atctcctcgacattttcagtctcccacccacggactttagagaagatcct
tgcctcagtttcatttccaaagatgtagtccgcatacggaagaaccttct
cttgggcatcacggaaaaactcacagataaagggagcggagaggttcatc
aanaaaaccttgttatttgcagcagcatgctcagcaacaagctgaataga
ttcaggggacaccgtaaggaaaaagcagcaatgt
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: CD453220
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||