GrainGenes Sequence Report: NSFT03P2_Contig14190
Sequence
CD452621
Contig
Ta.26095.1.S1_at NSFT03P2_Contig14190
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC307762
NCBI UniGene
Ta.63105
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001048379.1 Os02g0794700 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
CS wheat etiolated seedling root normalized cDNA library
Tissue
Root
Developmental Stage
Five day old etiolated seedling
Data Source
genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title
WHE1115_G12_N23ZT CS wheat etiolated seedling root normalized cDNA library Triticum aestivum cDNA clone WHE1115_G12_N23, mRNA sequence.
Strain
lab_host E. coli DH10B
Clone
WHE1115_G12_N23
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Normalization protocol used was that of Soares'. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttttttttttttttctaacttcaagaatattttttaaattccccaa
cttcttaaaaatacatgaagccgttgtaggctggcgccacacggtagcat
aaacgtaggcacatctgcagttctgctccttaattcagtctccggtccat
ctttggctgagctcacaactggaaaacagatcctcccaatgtttcacatc
ttgatgactaaaggaagcagacctattgcataagtaaactttggctaaac
gaaattatatgcatgtgtgaagtttgatcaaatatgtgtccacttattct
gtaacccactacatatacaatacgtgggcactagtaaaatccccccttcc
cttgtgagatataacatcaggcaacttcctctccccctgcgtgccttcat
cttcgttgattcacgacgagttcctgtcaaaccattccaccatggtggag
acgccgaatccggtctccgacctgttcttgtggctccacaccggaccagc
aatgtctatgtggatccactgaactttctcatggacaaactgcttcagga
ac
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: NSFT03P2_Contig14190
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||