GrainGenes Sequence Report: CD452577

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Sequence

CD452577

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

wEST map position

CD452577

NCBI UniGene

Ta.26182

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Transcribed locus, weakly similar to NP_001062377.1 Os08g0539400 [Oryza sativa (japonica cultivar-group)]'

Keyword

EST

Species

Triticum aestivum

Cultivar

Chinese Spring

Chromosome

6BL

Clone Library

CS wheat etiolated seedling root normalized cDNA library

Tissue

Root

Developmental Stage

Five day old etiolated seedling

Data Source

genbank	Release 136, Jun 15 2003
genbank	Updated Nov 2006

Title

WHE1113_E08_I15ZT CS wheat etiolated seedling root normalized cDNA library Triticum aestivum cDNA clone WHE1113_E08_I15, mRNA sequence.

Strain

lab_host E. coli DH10B

Clone

WHE1113_E08_I15

Probe

MAG1582

WHE1113_E08_I15

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Normalization protocol used was that of Soares'. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

tttttttttttttttttaaaaactccgggccattgcccctatcaatgccg
aacactcctaacttgcggagattggtgagaccttctatctcttgtatgat
ggcattttcccattcaaggtgcacacatcgcagtgtgttcagggatttca
gttccccgatccctactggcaccttaacagcataagcttgtatgaccatc
gcgcaacaagaagcgaaaggtacacaacatgctagtgatggccgttcttc
tccgatatagaattcattgccagctttcagttggcatagcttgctaagct
tggcgatggttcttggcagcctcgttattcttgtatatttgatgtctagt
gtctcaagttgtcgcaggttacccactgaatctggcaggtaatatattcc
ttcacaccctcttagagaaaggtatctcagatgaagatgattcccaatgt
gctcaaggtgatgatcggctagatctttagttccttcgaggtccagcaca
cgaagaaatctcatcttgtcagaaatataaaatggcctccacttgccaaa
caccgtcaatgaccgtatacatgacaacttcatggtggcctcaaactgac
cttcgtctccatcccaattactgcttatggcaaggtgacggaatgtgcct
tggttgtttgagttgcatccttcctcc


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