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GrainGenes Sequence Report: CD452367

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Sequence	CD452367
Contig	Ta.3568.1.S1_at Ta.3568.1.S1_x_at NSFT03P2_Contig7889
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC325035
wEST map position	CD452367
NCBI UniGene	Ta.3568
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_193695.2 CYCT1;4; cyclin-dependent protein kinase [Arabidopsis thaliana]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	4DS
Clone Library	CS wheat unstressed seedling shoot normalized cDNA library
Tissue	Etiolated shoot
Developmental Stage	Five day old seedling
Data Source	genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title	WHE1056_G11_M22ZT CS wheat unstressed seedling shoot normalized cDNA library Triticum aestivum cDNA clone WHE1056_G11_M22, mRNA sequence.
Strain	lab_host E. coli DH10B
Clone	WHE1056_G11_M22
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Normalization protocol used was that of Soares'. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	ttttttttttttttcccgcatcacggaccagccgcccaacgtttccacaa taaaaacacatgaacatgtacgcgcagtgtatgggaaaccatattctcga atcacggttcagatgtcccaaaatggccagcaaggaccgagatagcagct cctgcaacacagaaaaaacaataggttttcaccctnggttgttgcattta gcccccaatgccactctgccgatcattctacccagggtggttatgaatac ttccttttatggcctcaacctcttaaggtccctctcgctgctgtgatcat ggctcctgtgctccttccgctttggactgccgggctcgagagaatggcac agcccaccctcctcggtgtcctctaccgtgtgagcgacgttctgatgatc cttgtttatcttttccactgcatccaccgctgatggcgacgtattgaggc cttcattgctagtggcagtgccatccctcgcttcggattgtgctatcctc tggtttgagccttgcctttcagggtcatgctggttatgtccatggcagtc tggcaagctcggttgtctggatgctgaatgatttttatgcgcaggaggct cctcagcagctccaggagctttctccagggcatgttgattggctaaacct gcagagctgcttttactatcattcccttga

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