GrainGenes Sequence Report: NSFT03P2

GrainGenes Sequence Report: NSFT03P2_Contig15048

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Sequence

CD373992

Contig

Ta.20650.1.S1_at

NSFT03P2_Contig15048

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Transcribed locus, moderately similar to NP_001065399.1 Os10g0563200 [Oryza sativa (japonica cultivar-group)]'

Keyword

EST

Species

Triticum aestivum

Cultivar

Sumai3

Clone Library

Sumai3 Wheat Fusarium graminearum infected spike cDNA library

Tissue

Spike

Developmental Stage

Adult plant

Data Source

genbank	Release 136, Jun 15 2003
genbank	Updated Nov 2006

Title

WHE2620_F07_L14ZT Sumai3 Wheat Fusarium graminearum infected spike cDNA library Triticum aestivum cDNA clone WHE2620_F07_L14, mRNA sequence.

Strain

lab_host E. coli SOLR

Clone

WHE2620_F07_L14

Probe

MAG3956

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20. No effort was taken; to identify ESTs of fungal origin from this library, thus this EST; could be of wheat or fungal origin.; Seq primer: T7 primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes were sprayed at anthesis with Fusarium graminearum. Total RNA, and poly(A) RNA were prepared and pooled from infected spike at 0, 6, 12, 24, 36 and 48 hours after inoculation, a cDNA library was made , and the cDNA clones were in vivo excised to give pBluescript phagemids in G. Muehlbauer lab at the University of Minnesota (Kruger, W.M., Muehlbauer, G.J., Pritsch, C., Vance, C.). The cDNA library should contain genes of both wheat and fungal pathogen origin. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes were sprayed at anthesis with Fusarium graminearum. Total RNA, and poly(A) RNA were prepared and pooled from infected spike at 0, 6, 12, 24, 36 and 48 hours after inoculation, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in G. Muehlbauer lab at the University of Minnesota (Kruger, W.M., Muehlbauer, G.J., Pritsch, C., Vance, C.). The cDNA library should contain genes of both wheat and fungal pathogen origin. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

tttttttttttttttttaccgcaaacgcctcataaattagctagtgcact
gatccataagattatcttacacactaggcagttttactttacttgctctt
acacacaaaatcgcaaagcacactcagcggaagcaacaagatgccaacca
agttctctaggaggaaatgccaaaattcgacgacgatagccgtcatgtcg
tctggttcatgccgaagccatggatatgggggaataaataggtcagctgc
tcttctccaccagtgaaaagtaggagtgctgtttgctcatcatcctttgc
ttctatgtggaaggccctgcttggtggagctccaagttgcgaaggacgat
ccgtgcaaagatcttcgcttcaatgggagctgttgctgcggctgctgccc
accgtaagacccgggacgccccggctttctgaagcagggccgcgtggtgc
acagcatgtcgacccgccggcatagtgaactgaagcagagcaaaagctgc
acaagcacgaagaactgatgaaggattccgtagcgtgtcaacgaatctag
caatctcagctcggctgcatctaagatgtccagcttcttgtataaaaagt
gcgtcagcaacctgagataaggctgctgcgcctgaggatgaagcagccgt
agaaaacatttgtggatctgaaaaggtcaacacaaaggcttctatatgct
taaatgccaaccttcttgctccctcaaattttacaattttggaacta


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