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GrainGenes Sequence Report: Ta.25656.1.A1_at

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Sequence	CD373724
Contig	Ta.25656.1.A1_at
External Databases	Data at GenBank Data at EMBL Data at DDBJ
NCBI UniGene	Ta.25656
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus'
Keyword	EST
Species	Triticum aestivum
Cultivar	Sumai3
Clone Library	Sumai3 Wheat Fusarium graminearum infected spike cDNA library
Tissue	Spike
Developmental Stage	Adult plant
Data Source	genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title	WHE0483_c02_e03zT Sumai3 Wheat Fusarium graminearum infected spike cDNA library Triticum aestivum cDNA clone WHE0483_c02_e03, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE0483_c02_e03
Probe	MAG3732
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20. No effort was taken; to identify ESTs of fungal origin from this library, thus this EST; could be of wheat or fungal origin.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes were sprayed at anthesis with Fusarium graminearum. Total RNA, and poly(A) RNA were prepared and pooled from infected spike at 0, 6, 12, 24, 36 and 48 hours after inoculation, a cDNA library was made , and the cDNA clones were in vivo excised to give pBluescript phagemids in G. Muehlbauer lab at the University of Minnesota (Kruger, W.M., Muehlbauer, G.J., Pritsch, C., Vance, C.). The cDNA library should contain genes of both wheat and fungal pathogen origin. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes were sprayed at anthesis with Fusarium graminearum. Total RNA, and poly(A) RNA were prepared and pooled from infected spike at 0, 6, 12, 24, 36 and 48 hours after inoculation, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in G. Muehlbauer lab at the University of Minnesota (Kruger, W.M., Muehlbauer, G.J., Pritsch, C., Vance, C.). The cDNA library should contain genes of both wheat and fungal pathogen origin. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	ttttttttttttttttttttttattggctgaacagcaaacatctcatata ctacagtggtacagtggtggcaagtggggcaagtcagcaaggccaaccca ttttttcggagaaaacttccgatctattcatctttagaaagcttctgaat gatactactactaatcaacaggacatacatctgccccgcaggaaagaaaa aggaacaagacatacatctgcacaatatagtctgataagacaagaaagac atgatcacttctctgaagttgtagtcgggtacaaacgaacctgaaactca gtataaaaacagtataatttgaacggttgagtggaaaaacgccacgacag ggttggatctcgcgattttcaggaagtaatacatgtggtccttgcgcccc agctcatacagcacgaccttctcactgcctcacgcgctgccctggagcag ctcgacgtatatgcgcgccctgtccctgagggggtccaactcggccaccg tcatcagcgcctgtcggcagccgagcaccgcccactcctagataatgatt aatggagttactcctgtttcaggttgctcaacaagaaggttgcaaatggc atcacacattgccttgcgagaagcttcagccatagcaaatccaacataac cagtttcatggggagacacacagacatcaacaacctcaccagtctcttct tc

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