GrainGenes Sequence Report: CD057139

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Sequence

CD057139

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Hordeum vulgare

UniGene title 'CDNA clone: FLbaf97j10, mRNA sequence'

Keyword

EST

Species

Hordeum vulgare

Cultivar

Ingrid BC mlo-5

Clone Library

Tissue

leaf epidermis, 6 h and 24 h post inoculation with Blumeria graminis

Developmental Stage

7 d after germination

Data Source

genbank	Release 136, Jun 15 2003
genbank	Updated Nov 2006

Title

HO14E07S HO Hordeum vulgare cDNA clone HO14E07 5-PRIME, mRNA sequence.

Strain

lab_host XL10-Gold

Clone

HO14E07

Probe

BaH56C11

Remark

DB_xref: taxon:4513

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Patrick Schweizer; Transcriptome Analysis, Cytogenetics Department; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, D-06466 Gatersleben, Germany; Tel: 0049 (0)39482-5660; Fax: 0049 (0)39482-5595; Email: schweiz@ipk-gatersleben.de; Insert Length: 532 Std Error: 0.00; Plate: 14 row: E column: 7; Seq primer: SK.

Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Approximately 5 % of the clones correspond to cDNA from the fungi B. graminis hordei and tritici, respectively. Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1.2 kb

DNA

cggcacgagggttgctgtcatccttgttactgtgctcttcagaaccattt
tcggtggcaagaaggcagtggtatgttatatgttattttccgacatgtgc
cattgctgtatttcttaaaacctgagtcaaacgactgaccgcaacttctg
cttctttcaggcacctgtgaagcccgctgctgtggtgaagaagcccagtg
ccacggaagccgatgccgccggaagcagcggtgacaaggaggacaaggag
gatgagaaggatgacacggctgcaccacgcagaaggtcgcgaagggagac
gtaggccagaccttcacattttcggacgtctcggtcctcgcctttttgtg
aactcctcgagtgtataatcgaacagtcttgccgccgcttctgctgctac
ccctagttttaagtcactagaatctacgagccggtgaagctttgtgttgt
ttgtgtgatgtcctgaatcagctagaagtgattttctttttcttttgtat
caaatgaaacttgaaaggatcttttgatattt


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