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GrainGenes Reference Report: PHP-82-419

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Reference	PHP-82-419
Title	Ethidium bromide preferentially releases high mobility group chromosomal proteins HMGc and HMGd from wheat embryo nuclei
Journal	Physiologia Plantarum
Year	1991
Volume	82
Pages	419-422
Author	Arwood L Hill E Spiker S
Abstract	The High Mobility Group (HMG) chromosomal proteins have been implicated as structural proteins of transcriptionally poised chromatin in animal systems Despite the suspected functions of these proteins, no bioassay for them exists and they are defined operationally (by the procedures used to isolate and purify them) Plant systems have chromosomal proteins that can be operationally defined as HMG proteins However, because of the lack of suitable bioassays and because the plant HMG proteins are structurally distinct from their putative animal counterparts, no clear relationship between plant and animal HMG proteins has been established One diagnostic characteristic of vertebrate animal HMG proteins is that a subset of them is released from chromatin by intercalating agents that affect the topology of DNA We show here that wheat (Triticum aestivum) embryo HMGc and HMGd (but not HMGa and HMGb) are preferentially released from nuclei treated with 75 mM ethidium bromide. Because vertebrate animal HMG14/17 (but not HMG1/2) displays this characteristic, the observation suggests that these wheat chromosomal proteins may be the plant analogs of vertebrate HMG14/17.
Keyword	chromatin [ Show all 7 ]

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