GrainGenes Reference Report: PEU-04-223
Reference
PEU-04-223
Title
A retrotransposon sequence is related to DNA instability in barley microspore culture
Contained in
(2004) Genetic variation for plant breeding. Proceedings of the 17th EUCARPIA General Congress, Tulln, Austria, 8-11 September 2004.
Year
2004
Pages
223-225
Author
Munoz M
[ Show all 8 ]
Abstract
To assay gametoclonal variation in barley, a set of doubled haploid (DH) plants (cv. 'Igri') regenerated from isolated microspore culture by direct embryogenesis, was especially developed. AFLP analysis was performed with eleven enzyme-primer combinations, that represented the study of 719 band polymorphisms. Only one change in the banding pattern was observed, indicating a high degree of genetic stability. An extra band was present in DH lines regenerated from microspores, treated or not with sodium azide, representing a 'hot spot' of instability. The sequence of this band was related to barley 'sukkula' retrotransposon element. Our preliminary results suggest the activation of retrotransposon by barley isolated microspore culture
Keyword
barley
[ Show all 14 ]
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: PEU-04-223
|
| ||||
|
| ||||
|
| ||||
|
| ||||
|
| ||||
|
| ||||
|
| ||||
|
|
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||