GrainGenes Reference Report: PEU-04-199
Reference
PEU-04-199
Title
A comparison of genetic stability in haploid plants derived from wheat x maize crossing and anther culture using molecular markers
Contained in
(2004) Genetic variation for plant breeding. Proceedings of the 17th EUCARPIA General Congress, Tulln, Austria, 8-11 September 2004.
Year
2004
Pages
199-201
Author
Badraldin-Ebrahim S Tahereh M Vollmann J Grausgruber H Ruckenbauer P
Abstract
The utility haploid-culture methods depends on the amount of genetic stability and the aim of the study. In this study, RAPD and AFLP techniques were used to evaluate the genetic stability of produced haploids via wheat x maize crossing and anther culture methods. None of the six random primers used in PCR reactions showed detectable polymorphic bands in haploid plants derived from two techniques. Most of the paired primers used in AFLP technique performed polymorphisms among the haploids. Analysis of the results indicated that the frequency of polymorphic bands varied from 2.6 to 7.8% among anther-regenerated plants whereas this frequency was 2.6 to 3.3% among haploids derived from wheat x maize crossing. These results indicated that RAPD analysis is not sufficiently sensitive to detect genetic changes that occur during haploid production from two methods. The results also showed that utilization of wheat x maize crosses might be more efficient than anther culture for producing stable haploid plants
Keyword
agriculture
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GrainGenes Reference Report: PEU-04-199
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||