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GrainGenes Reference Report: PEU-04-177

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Reference	PEU-04-177
Title	DNA sequence polymorphisms and their application in bread wheat
Contained in	(2004) Genetic variation for plant breeding. Proceedings of the 17th EUCARPIA General Congress, Tulln, Austria, 8-11 September 2004.
Year	2004
Pages	177-180
Author	Ravel C [ Show all 17 ]
Abstract	single-nucleotide polymorphisms (snps) constitute an abundant source of dna polymorphisms, which have been successfully used to identify loci that are associated with a particular phenotype. information on such markers in plants is still scarce. for bread wheat, snp data are restricted to few genes. this can be explained by the hexaploidy of triticum aestivum making difficult snp discovery. we developed a novel method for snp discovering in bread wheat. this strategy was based on the development of highly specific pcr-primers used to sequence 27 lines. snps were discovered from sequence alignment. some of them were genotyped by mass spectrometry in a collection of approx equal to 155 lines, which were both evaluated for agronomic traits and genotyped at 42 neutral microsatellites. traits estimated were protein content, the quantity of high molecular weight glutenins and that of the glu-b1x subunit. neutral markers were used to infer population structure, further included in linear models for association studies. the results showed 89 snps in approximately 20 kpb, i.e. one snp every 223 bp on average. among snps genotyped, 3 snps were located along the glu-b1-1 gene, two of them in the promoter and one in the coding sequence, while 3 nonsynonymous snps were located along the gene coding for transcriptional factor spa (storage protein activator). in both years, snps from glu-b1-1 had a significant effect on the studied variables, whereas those of spa had no effect. such results might indicate that some haplotypes for glu-b1-1 were linked with higher protein content through an increased amount of high molecular weight glutenins, especially the subunit glub1x
Keyword	dna [ Show all 18 ]

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