Query (optional)   in Class  

GrainGenes Reference Report: PEU-04-49

[Submit comment/correction]

Reference
PEU-04-49
Title
Development and use of genomic tools for cereal introgression breeding
Contained in
Reference(2004) Genetic variation for plant breeding. Proceedings of the 17th EUCARPIA General Congress, Tulln, Austria, 8-11 September 2004.
Year
2004
Pages
49-51
Author
Hernandez P
Vollmann J
Grausgruber H
Ruckenbauer P
Abstract
There is an increasing amount of public model sequence information in model plants available with potential use in agriculture. In cereals, the complete sequence of the rice genome is the basic resource. Additionally, there is an increasing amount of sequence information for the main cultivated species, such as wheat and barley. Wild relatives with introgression potential can also benefit from such resources through a comparative genomic approach. One of such species is Hordeum chilense. It exhibits great levels of polymorphism and high crossability with different cereal genera. In addition, interesting biotic and abiotic stress resistance genes, and important quality traits like carotene content and seed storage protein variability shown in the species are also expressed on wheat backgrounds and are the basis of a breeding programme. In the past years, the search for the most suitable DNA marker system for tagging H. chilense genomic regions in a wheat background has lead to the development of RAPD and SCAR markers for this species. RAPDs represent an easy way of quickly generating suitable introgression markers. SCARs are more specific assays, suitable for automation or multiplexing. Direct sequencing of RAPD products is a cost-effective approach that reduces labour and costs for SCAR development. Transfer of SSTs from wheat and barley have also been useful approaches. More recently, SNP development is being accomplished for the species. A practical application of the different marker approaches has been the generation of derived introgression products
Keyword
agriculture
[ Show all 21 ]

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.