GrainGenes Reference Report: PEU-04-35
Reference
PEU-04-35
Title
Efficiency of different PCR-based marker systems in assessing genetic diversity among rye inbred lines
Contained in
(2004) Genetic variation for plant breeding. Proceedings of the 17th EUCARPIA General Congress, Tulln, Austria, 8-11 September 2004.
Year
2004
Pages
35-39
Author
Bolibok H
[ Show all 6 ]
Abstract
The aim of this study was to investigate the efficiency of ISSR, SSR and SAMPL marker systems in detecting genetic polymorphism among 30 rye inbred lines. Each marker system was able to discriminate among the materials analysed with the lowest value of average genetic similarity (GS) obtained with ISSR markers (0.2888) and the highest with SAMPLs (0.5381). EST-derived SSRs turned out to be less efficient in detecting genetic diversity than those from genomic libraries (average GS values 0.3814 and 0.3221, respectively). The average GS value for combined SSR data was 0.3569. The lack of correlations between similarity and cophenetic matrices obtained with different marker systems suggests that different systems should be used simultaneously for a genetic diversity study to exploit as many sources of polymorphism as possible
Keyword
analytical methods
[ Show all 12 ]
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: PEU-04-35
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||