GrainGenes Reference Report: PCT-77-203
Reference
PCT-77-203
Title
Biolistic transfer of the gene uidA and its expression in haploid embryo-like structures of triticale (xTriticosecale Wittmack)
Journal
Plant Cell Tissue and Organ Culture
Year
2004
Volume
77
Pages
203-209
Author
Rubio S Jouve N Gonzalez JM
Abstract
Summary: Haploid embryo-like structures (ELS) of triticale were obtained by in vitro androgenesis. These structures were bombarded with gold microparticles 1 micrometer in diameter coated with the plasmid pAHGUS, using the Dupont PDS He/1000 apparatus. Analysis was made of the influence of genotype, duration of ELS pre-culture, helium pressure and shooting distance on the transfer and expression of the gene uidA. No significant differences were seen between genotypes or duration of pre-culture; differences were found, however, with respect to helium pressure and shooting distance. The combination of 1100 psi helium and a 6 cm shooting distance led to the greatest mean number of uidA expression foci in the ELS (1.63), and the greatest mean number of ELS showing at least one focus (0.38). With a pressure of 1800 psi and a 9 cm distance, only 0.35 foci were obtained per ELS, and only 0.18 ELS had one or more foci. These results are being used as references in a program for obtaining double haploid and transgenic triticale plants
Keyword
agrobacterium tumefaciens
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GrainGenes Reference Report: PCT-77-203
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||