GrainGenes Reference Report: PCT-58-119
Reference
PCT-58-119
Title
Plant regeneration from protoplasts of Triticum aestivum L. cv. Nakasoushu
Journal
Plant Cell, Tissue and Organ Culture
Year
1999
Volume
58
Pages
119-125
Author
Li H Machii H Hagio T Takezaki A Hirabayashi T
Abstract
A fast-growing, small, granular, embryogenic callus was selected from primary calli induced from the Japanese wheat cultivar Nakasoushu and the Australian wheat cultivar Bodallin Regenerable and fine suspension cultures were induced three to six months after liquid culture was initiated and were characterized by dense cytoplasm and active division These suspension cultures routinely provided high yields of protoplasts with about 90% viability when incubated in a modified KMP (Kao and Michayluk, 1975) medium containing 1 mg/l 2,4-D (2,4-dichlorophenoxyacetic acid), and 1 mg/l zeatin Nakasoushu and Bodallin protoplasts divided at frequencies of 8.6% and 11.1%, respectively, in agarose-solidified media When Nakasoushu protoplasts were cultured with effective nurse cells of sorghum and wheat, protoplast division increased to 16.9% and 12.6%, respectively. Plating efficiencies varied from 0.03% to 2.5%. After subculture, protocolonies yielded embryogenic calli and somatic embryos, from which green plants were eventually regenerated. Whole plants obtained from Nakasoushu protoplasts were fertile, demonstrating the first report of Japanese cultivars in wheat protoplast cultures.
Keyword
callus
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GrainGenes Reference Report: PCT-58-119
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||