GrainGenes Reference Report: PBR-122-83
Reference
PBR-122-83
Title
Amplified fragment length polymorphism-derived microsatellite sequence linked to the Pch1 and Ep-D1 loci in common wheat
Journal
Plant Breeding
Year
2003
Volume
122
Pages
83-85
Author
Groenewald JZ Marais AS Marais GF
Abstract
Summary: Amplified fragment length polymorphism (AFLP) markers linked to the Aegilops ventricosa-derived chromosome segment in 'VPM1' on which the eyespot resistance gene, Pch1, and the endopeptidase gene, Ep-D1b, occur were identified. One marker was isolated from the gel, cloned and sequenced. Sequence analysis revealed a microsatellite repeat motif. Sequence-specific primers were designed to amplify a product containing the repeat motif, and the microsatellite marker was tested for cosegregation with the Ep-D1b allele. Distinct alleles were produced by the Pch1 sources, normal wheat and wheat containing the Lr19 translocation. A recombination frequency of 0.02 was calculated between the microsatellite marker and Ep-D1
Keyword
aegilops
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GrainGenes Reference Report: PBR-122-83
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||