GrainGenes Reference Report: PBR-121-400
Reference
PBR-121-400
Title
Identifying AFLP and microsatellite markers for vernalization response gene Vrn-B1 in hexaploid wheat using reciprocal mapping populations
Journal
Plant Breeding
Year
2002
Volume
121
Pages
400-406
Author
Barrett B Bayram M Kidwell K
Abstract
Summary: Marker-assisted selection may be useful for combining specific vernalization response (Vrn) alleles into a single wheat genotype for yield enhancement; however, DNA markers are only available for two of the three genes identified to date. The objectives of this study were to investigate reciprocal effects on days to heading using F2 populations generated by cross-hybridizing near-isogenic lines (NILs) carrying spring (Vrn-B1; TDB) and winter (vrn-B1; TDC) alleles, and to identify markers linked to Vrn-B1 through genetic linkage analysis. Heading data were recorded for 91 and 89 progeny from reciprocal mapping populations TDB/TDC and TDC/TDB, respectively, and significant (P < 0.0001) reciprocal and dominance effects were detected. Among 207 amplified fragment length polymorphisms primer pairs and seven wheat microsatellite markers screened, two and one, respectively, were linked distally to Vrn-B1 on wheat chromosome 5BL. Microsatellite Xgwm408 was most closely linked to Vrn-B1 at 3.9 and 1.1 cM in the TDB/TDC and TDC/TDB map, respectively. Reciprocal differences in recombination distances emphasize the importance of female parent choice when generating mapping populations. Molecular markers are now available for three Vrn loci in wheat
Keyword
aflp
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: PBR-121-400
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||