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GrainGenes Reference Report: PAG-98-87

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Reference	PAG-98-87
Title	Species-specific PCR markers for sugarcane and related taxa
Contained in	(1998) Plant & Animal Genome VI.
Year	1998
Pages	87
Remark	Poster P44
Author	Pan Y-B Burner DM
Type	Abstract
Abstract	The objective of this study was to develop species-specific PCR markers for F1 progeny selection in sugarcane breeding. Generic primers P1 and P2 primed DNA products from the 5S ribosomal DNA spacer were obtained from elite sugarcane varieties and several related species including Saccharum officinarum, S. spontaneum, Erianthus spp., three cytotypes (2n = 30, 60, and 90) of S. giganteum, Miscanthus sinensis, maize, and sorghum. The PCR products exhibited some length polymorphisms and Southern blot experiments indicated that PCR products from various taxa did not always cross hybridize. These PCR products were cloned into a plasmid vector and sequenced. We will discuss sequence variations among these taxa and the prospects for developing species-specific PCR markers to screen for hybrids among inter-taxa crosses.
External Databases	http://www.intl-pag.org/pag/6/abstracts/267.html
Sequence	5SrRNA Green German [ Show all 24 ]

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