GrainGenes Reference Report: MYR-113-523
Reference
MYR-113-523
Title
Proteomic identification of extracellular proteins regulated by the Gna1 Galpha subunit in Stagonospora nodorum.
Journal
Mycological Research
Year
2009
Volume
113
Pages
523-531
Author
Tan KC Heazlewood JL Millar AH Oliver RP Solomon PS
Abstract
The fungus Stagonospora nodorum is the causal agent of stagonospora nodorum blotch (syn. leaf and glume blotch) disease of wheat. The Gna1-encoded Galpha protein is an important signal transduction component in the fungus, which is required for full pathogenicity, sporulation and extracellular depolymerase production. In this study, we sought to gain a better understanding of defects associated with the gna1 mutant by using two-dimensional gel electrophoresis to analyse the extracellular proteome for differences to the wildtype. Mass spectrometry analysis of altered abundant protein spots and peptide matching to the Stagonospora nodorum genome database have led to the identification of genes implicated in cell wall degradation, proteolysis, RNA hydrolysis and aromatic compound metabolism. In addition, quantitative RT-PCR has demonstrated that some of the encoding genes showed differential expression throughout host infection. Implications of these proteins and their corresponding genes in fungal virulence are discussed.
External Databases
http://dx.doi.org/10.1016/j.mycres.2009.01.004
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: MYR-113-523
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||