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GrainGenes Reference Report: MGG-217-355

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Reference	MGG-217-355
Title	Nucleotide sequence of a complementary DNA coding for the NADPH-protochlorophyllide oxidoreductase PCR of barley Hordeum-vulgare L. and its expression in Escherichia-coli
Journal	Molecular and General Genetics
Year	1989
Volume	217
Pages	355-361
Author	Schulz R [ Show all 8 ]
Abstract	The primary structure of the NADPH-protochlorophyllide oxidoreductase of barley has been deduced from the nucleotide sequence of a cloned full-length cDNA. This cDNA hybridizes to a 1.7 kb RNA whose steady-state level in dark-grown seedlings is drastically reduced upon illumination. The predicted amino acid sequence (388 residues in length) includes a transit peptide of 74 amino acids whose end point has been delimited by sequencing the N- terminus of the mature protein. Expression of the cDNA in Escherichia coli leads to the synthesis of an enzymatically active precursor of the NADPH-protochlorophyllide oxidoreductase. Activity of this protein in bacterial lysates is completely dependent on the presence of NADPH and protochlorophyllide and requires light.
Probe	pHvDF1 pHvA4 pHvA7
Keyword	Amino acid sequence Light regulation Molecular sequence data

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