GrainGenes Reference Report: MBR-7-163
Reference
MBR-7-163
Title
Accelerated production and identification of fertile, homozygous transgenic wheat lines by anther culture
Journal
Molecular Breeding
Year
2001
Volume
7
Pages
163-173
Author
Massiah A Rong HL Brown S Laurie S
Abstract
Summary: Anther culture has been developed in the winter wheat cultivar Florida to achieve accelerated production and identification of homozygous transgenic lines. With untransformed, seed-derived plants to develop the culture system, it was shown that cold pre-treatment of spikes excised from donor plants and addition of 2,4-dichlorophenoxyacetic acid together with either kinetin or 6-benzylaminopurine in the callus induction medium improves the anther culture response. The procedure developed allowed production of fertile homozygous lines within 8-9 months, which includes an 8-week vernalisation period. With transgenic wheat plants produced by particle bombardment as donors, we show that the system can be used to produce homozygous transgenics, requiring one generation cycle. Both T0 tissue culture-derived plants and their T1 seed-derived descendents serve as suitable donors. We show that an anther culture response comparable to that of untransformed, seed-derived plants can be achieved with T0 tissue culture-derived plants. PCR and Southern molecular analyses of anther culture-derived transgenics show that the transgenes are stably inherited; there are no perturbations at the chromosomal level around the sites of transgene integration as a result of in vitro chromosome manipulation during anther culture
Keyword
2,4-dichlorophenoxyacetic acid
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GrainGenes Reference Report: MBR-7-163
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||