GrainGenes Reference Report: MBR-5-579
Reference
MBR-5-579
Title
PCR detection of genetically modified soya and maize in food stuffs
Journal
Molecular Breeding
Year
1999
Volume
5
Pages
579-586
Author
Hurst CD Knight A Bruce IJ
Abstract
Summary: The detection of genetically modified foodstuffs is becoming both a food sales and legal necessity. This study reports a rapid DNA extraction/PCR-based method for the detection of genetically modified soya (GMS) and maize (GMM) in mixed samples of transgenic and unmodified soybeans and maize kernels, and a variety of processed samples including soya flour, soya protein isolates, extruded defatted soya, acid- and alcohol-precipitated soya concentrates, soya lecithin, maize grits, seasoned corn puffs and salted corn chips. The presence of GMS DNA was determined with two pairs of primers directed towards different GMS target sequences and GMM by one primer pair. In addition, a multiplex PCR reaction which utilises an internal positive control was developed for both genetically modified organisms (GMOs). Results indicated that the methods are sensitive and specific enough to detect GMS down to a level of 0.01% dry weight in single-product PCRs and 0.1% in multiplex PCRs and GMM down to 0.001% dry weight in single-product PCRs and 0.01% in multiplex PCR. The methods are considered to represent a viable route for the commercial detection of GMS and GMM in foodstuffs
Keyword
alkyl (aryl) transferases
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GrainGenes Reference Report: MBR-5-579
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||