GrainGenes Reference Report: JFC-48-186
Reference
JFC-48-186
Title
Protein insolubilization and thiol oxidation in sulfite-treated wheat gluten films during aging at various temperatures and relative humidities
Journal
Journal of Agricultural and Food Chemistry
Year
2000
Volume
48
Pages
186-192
Author
Morel MH Bonicel J Micard V Guilbert S Morel M-H
Abstract
Summary: Gluten films were prepared by casting an acidic and ethanolic solution of gluten previously treated with sodium sulfite. The effects of sulfitolysis on proteins were investigated by SE-HPLC and thiol/ disulfide content measurements. During sulfitolysis, insoluble glutenin macropolymer was converted into its constitutive subunits. About 10% of gluten disulfide bonds were cleaved, of which three-fourths originated from interchain disulfide bonds. Oxidation of thiol groups released during sulfitolysis was followed for various temperatures (T) and relative humidities. Oxidation was shown to be a second-order rate process occurring below the glass transition temperature (Tg) and related to T-Tg. Thiol oxidation ensured the formation of interchain bonds between specific classes of gluten proteins according to an ordered process. Intrachain bonds were also formed and through thiol/disulfide-exchange reactions were finally converted to interchain bonds. Thus, fully oxidized gluten films had more insoluble glutenin macropolymers than native gluten
Keyword
aging
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GrainGenes Reference Report: JFC-48-186
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||