GrainGenes Reference Report: JFC-47-5261
Reference
JFC-47-5261
Title
Real-time quantitative PCR detection of genetically modified maximizer maize and roundup ready soybean in some representative foods
Journal
Journal of Agricultural and Food Chemistry
Year
1999
Volume
47
Pages
5261-5266
Author
Vatlingom M Pijnenburg H Gendre F Brignon P Vaitilingom M
Abstract
Summary: A fast and quantitative method was developed to detect transgenic 'Maximizer' maize 'event 176' (Novartis) and 'Roundup Ready' soybean (Monsanto) in food by real-time quantitative PCR. The use of the ABI Prism 7700 sequence detection system allowed the determination of the amplified product accumulation through a fluorogenic probe (TaqMan). Fluorescent dyes were chosen in such a way as to coamplify total and transgenic DNA in the same tube. Using real-time quantitative PCR, 2 pg of transgenic or total DNA per gram of starting sample was detected in 3 h after DNA extraction and the relative amounts of 'Maximizer' maize and 'Roundup Ready' soybean in some representative food products were quantified
Keyword
alkyl (aryl) transferases
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GrainGenes Reference Report: JFC-47-5261
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||