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GrainGenes Reference Report: JFC-42-2460

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Reference	JFC-42-2460
Title	Fractionation of wheat, barley, and rye prolamins by cation exchange FPLC
Journal	Journal of Agricultural and Food Chemistry
Year	1994
Volume	42
Pages	2460-2465
Author	Chirdo F Fossati C Anon M
Abstract	Wheat, rye, and barley prolamins were separated using optimized elution conditions by cation exchange FPLC on an analytical Mono-S column Wheat gliadins were resolved into a larger number of peaks than has been obtained hitherto Most fractions contained a mixture of components as determined by SDS-PAGE and A-PAGE At least one fraction was obtained for each group of gliadins that contains only one major component with minimal contamination by other components Only omega-gliadins occurred in five of the fractions A preparative S-Sepharose Fast Flow column, run under similar chromatographic conditions was used to isolate omega-gliadins This procedure could be used as the first step for the purification of individual gliadin components. Chromatography on the Mono-S column was highly reproducible and showed promise as a means of differentiating wheat cultivars. Furthermore, FPLC was applied to fractionate prolamins from barley and rye.
Keyword	barley [ Show all 11 ]

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