GrainGenes Reference Report: APT-34-352
Reference
APT-34-352
Title
Expression of Barley yellow dwarf virus-GPV ORF4 in E. coli and preparation of its specific antiserum
Journal
Acta Phytopathologica Sinica
Year
2004
Volume
34
Pages
352-355
Author
Li Y Wu M Wang Z Zhang W Cheng Z
Abstract
According to published nucleotide sequence, the open reading frame 4 (ORF4) gene of barley yellow dwarf virus GPV (BYDV-GPV) was synthesized by reverse transcription-polymerase chain reaction and inserted into expression vector pET-5a for overexpression in prokaryotic cells. The result of SDS-PAGE showed that specific expression of a 17 kDa protein was achieved after IPTG induction. The expressed protein was purified from SDS-PAGE gel and the antiserum against BYDV-GPV 17 kDa protein was raised in rabbit. The antiserum could be used for specific detection of the 17 kDa protein in oats infected by BYDV-GPV
Keyword
barley
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: APT-34-352
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||