GrainGenes Reference Report: JCS-33-163
Reference
JCS-33-163
Title
Chromosomal control of albumins and globulins in wheat grain assessed using different fractionation procedures
Journal
Journal of Cereal Science
Year
2001
Volume
33
Pages
163-181
Author
Singh J Skerritt JH
Abstract
Summary: A systematic study of the water-soluble (albumin) and salt-soluble (globulin) proteins of wheat flour, by analysis of null genetic lines using different methods of protein separation, has established the location of several of their genes on individual chromosomes. SDS-PAGE analysis of water-soluble proteins indicated the chromosomal location of polypeptides of Mr 64000, Mr 62000 and Mr 16000 on 4DL, 4BS and 3DS respectively. Similarly, two globulin polypeptides of Mr 38000 and Mr 39000 were assigned to chromosomes 2A, 2B, 2D, 3BS, 3DS, 4DS, 5DL, 6DS, 7BS or 7DL using isoelectric focusing. In addition, several non-gluten proteins have been mapped on chromosome 1D, 3AL, 3BS, 3DS, 4AL, 4BS, 4DS, and 5DL by reversed-phase HPLC (RP-HPLC). The proteins separated by RP-HPLC were also correlated with proteins resolved with other methods. The polypeptides assigned to different chromosomes using SDS-PAGE and isoelectric focusing were also analysed by 2-D electrophoresis to confirm the chromosomal location of genes controlling expression of particular polypeptides. Some other proteins that were not resolved by either method alone, were able to be mapped on chromosomes using 2-D electrophoresis. The chromosome-specific proteins are now being used to develop chromosome-specific markers, which may be valuable to discover spontaneous deletions as well as natural translocations from alien species
Keyword
2d electrophoresis
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GrainGenes Reference Report: JCS-33-163
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||