Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: JCS-30-227

[Submit comment/correction]

Reference	JCS-30-227
Title	Occurrence and stabilities of oat trypsin and chymotrypsin inhibitors
Journal	Journal of Cell Science
Year	1999
Volume	30
Pages	227-235
Author	Mikola M Mikkonen A
Abstract	Nine chymotrypsin and four trypsin inhibitors have been extracted and separated from ungerminated oat grains The inhibitors fell into two groups, based on their heat and pH stabilities Members of the most abundant group are labile and are inactivated at 80 degrees C or at pHs of 3.3 or lower Members of the second group are stable and are resistant to boiling for 30 min On germination, the labile inhibitors are inactivated after 2 days and the stable chymotrypsin inhibitors after 3 days Most of the labile inhibitors from ungerminated grain are destroyed when incubated at 20 degrees C for 20 h but addition of PMSF, a serine protease inhibitor, prevented their inactivation. Labile inhibitors in extracts of ungerminated oats are inactivated on incubation with an extract prepared from germinated oats, but not in the presence of PMSF. Most oat chymotrypsin and trypsin inhibitors are heat labile and pH sensitive. These inhibitors are apparently inactivated by serine proteinase(s) already present in ungerminated grain.
Keyword	avena sativa [ Show all 14 ]

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.